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Direct effect of immunosuppressants on MSC functions in vitro. MSCs were loaded into a 96-well plate at 1 × 104 cells/well and treated with immunosuppressants for 24 h. Viability was measured by XTT assay (a) and the levels of TGF-β1 (b) and PGE2 (c) in culture medium were measured by ELISA.
