Figure 5.

miR‐374a’s oncogenic activity is in part through negative regulation of LACTB in vitro. A, LACTB protein expression was decreased after transfected with siRNA targeting LACTB (LACTB‐siRNA) or siRNA negative control (NC) in MDA‐MB‐231 and MCF‐7 cells. B, Proliferation assays indicated that downregulation of miR‐374a inhibited the proliferation capacity of MDA‐MB‐231 and MCF‐7 cells, which was partially restored when LACTB was inhibited by RNA interference. C, EdU assays indicated that compared with negative control groups, miR‐374a knockdown significantly inhibited proliferative activity, which was significantly reversed by the LACTB silencing. *P < .05, **P < .01