Figure 2.

linc‐UFC1 promotes cell proliferation, migration, and invasion and inhibits apoptosis of cervical cancer cells. A, Three shRNAs targeting linc‐UFC1 were tested for their knockdown efficiency by qRT‐PCR. B, The cell proliferation induced by linc‐UFC1 shRNA was tested by CCK‐8 assay. C, The cell proliferation induced by linc‐UFC1 shRNA was tested by colony formation assay. D, Cervical cancer cells with linc‐UFC1 knockdown were stained with a combination of annexin V and 7‐AAD and analyzed by FACS. Cells positive for annexin V staining were counted as apoptotic cells, and the percentage of apoptotic cells is shown. E, The effect of linc‐UFC1 on cell cycle distribution was detected by FACS assay. F, The migration and invasion of control and linc‐UFC1‐silencing cells were determined by Transwell assay. Data are shown as mean ± SD; *P < .05