Figure 5.

linc‐UFC1 directly associates with miR‐34a. A, Hela cell lysates were incubated with biotin‐labeled linc‐UFC1, and the pull‐down miR‐34a was assessed by qRT‐PCR. B, The RIP assay was performed to pull down the miR‐34a interacted with linc‐UFC1 or mutant linc‐UFC1. C, Luciferase activity in Hela cells cotransfected with miR‐NC or miR‐34a and luciferase reporters containing wild‐type or mutant linc‐UFC1. D, Anti‐AGO2 RIP was performed in Hela cells with overexpression of miR‐NC or miR‐34a and followed by qRT‐PCR to detect linc‐UFC1 associated with AGO2. E, The relative expression of miR‐34a in wild‐type or mutant linc‐UFC1 overexpressed Hela cells. F, The cell proliferation was determined by CCK‐8 assay in the indicated clones. G, The cell migration and invasion were determined by Transwell assay in the indicated clones. Data are shown as mean ± SD; *P < .05