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Stability of RP11‐466I1.1 expression in serum. The expression of lncRNA in the specimen was calculated using the 2‐∆CT method relative to GAPDH. A, Three specific primers (a,b,c) were designed whose corresponding target fragments crossed different connection segments. B, Three kinds of target fragments were transcribed in each tissue specimen using the 3 specific primers, whose concentration levels were pretty consistent in the same specimen
