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. 2018 Jun 1;7(7):3057–3065. doi: 10.1002/cam4.1601

Figure 1.

Figure 1

Recombinant human CT83 determination. A, Purified recombinant plasmid pET28a‐CT83 (lane 2), and digested by Bgl II and Xho I (lane 1). B, The recombinant pET28a‐CT83 plasmid was transformed into E.coli BL21 (DE3) and a 14 kDa recombinant protein was induced by 0.5 mmol/L IPTG (lane 2), compared with the uninduced group (lane 1). And most of recombinant protein was detected on pellet (lane 4) but not supernatant (lane 3) of ultrasonic crushing fluid. C, Recombinant protein was purified by affinity chromatography. Lane 1: ultrasonic precipitation solution dissolved with Urea. Lane 2: flow liquid. Lane 3: 10 mmol/L imidazole. Lane 4: 20 mmol/L imidazole. Lane 5: 50 mmol/L imidazole. Lane 6: 200 mmol/L imidazole. Lane 7: 500 mmol/L imidazole. D, Determination of recombinant protein concentration by SDS‐PAGE. Lane 1: 1 μg BSA standard. Lane 2: 2 μg BSA standard. Lane 3: 4 μg BSA standard. Lane 4: 1 μL CT83 recombinant protein. Lane 5: 2 μL CT83 recombinant protein. M: Molecular weight of DNA (bp) or protein (kDa) marker