Figure 2.

Purity and titer analysis of purified CT83 mAbs 7G4. To create monoclonal antibodies against CT83, BALB/c mice were immunized with recombinant human CT83 protein, and hybridoma cell 7G4 was generated by using the iliac lymph node method. The hybridomas were screened for their activity to bind recombinant human CT83 protein by indirect ELISA. A, a SDS‐PAGE determinant of 7G4 antibody. Lane 1: 0 μL purified CT83 mAb 7G4. Lane 2: 5 μL purified CT83 mAb 7G4. Lane 3: 10 μL purified CT83 mAb 7G4. B, Titer detection of before purification CT83 mAb 7G4 in ascites. C, Titer detection of purified CT83 mAb 7G4