Figure 3.

Identification of the specificity of the purified CT83 mAb 7G4 by Western blotting and immunohistochemistry. A, Western blotting was employed to validate the specificity of CT83 mAb 7G4 in detection of endogenous human CT83 in RKO, SW1116, NCI‐H1299, HuH‐7, HeLa, MCF‐7, MDA‐MB‐231, HNE‐1, CNE‐2 cancer cell lines, and 293T cell line. B, Immunohistochemistry was employed to validate the specificity of CT83 mAb 7G4 in detection of endogenous human CT83 in lung cancer, nasopharyngeal cancer, cervix cancer, colorectal cancer, breast cancer, gastric cancer, melanoma, urothelial carcinoma, and endometrioid adenocarcinoma tissue (400×)