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CT83 binding activity of 7G4‐1‐Ga was analyzed by ELISA and flow cytometry. A, The reactivity of mAb 7G4, 7G4‐1‐Ga complex with CT83 was estimated by ELISA using CT83 coated plates. B, The CT83‐expressing cancer (MCF‐7, SW1116, RKO, CEN‐2) cells and CT83‐non‐expressing 293T cell were incubated with 7G4, 7G4‐1‐Ga, then with PE‐conjugated anti‐mouse IgG before flow cytometry analysis
