Figure 2.

NDR1 reduced expression in prostate cancer cell and tissues metastasis. A, mRNA expression in PC3 and PC3‐CTC cells and LNCaP and C4‐2 cells. GAPDH mRNA was used as an internal control. Each experiment was repeated 3 times. *P < .05, **P < .01 by Student's t test. B, Protein expression analysis in PC3 and CTCs cells and LNCaP and C4‐2 cells. β‐actin was used as an internal control. Each experiment was repeated 3 times. *P < .05, **P < .01 by Student's t test. C, Analysis of NDR1 protein expression by immunohistochemistry in primary and metastatic prostate cancer. Magnification images taken at 200× (red bar = 50 μm) and 400× (red bar = 20 μm). D, NDR1 expression in samples evaluated by IRS score, Mann‐Whitney test