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. 2018 Jun 14;7(7):3143–3156. doi: 10.1002/cam4.1523

Figure 5.

Figure 5

HOTAIRM1 was a target of miR‐148a in Fadu cells (A‐B) Prediction of binding sites of miR‐148a and HOTAIRM1 by bioinformatics (miRcode 11). Then luciferase activity analysis showed transfection of miR‐148a could significantly inhibit the HOTAIRM1 wild‐type luciferase gene carrier fluorescence activity. **P < 0.01 compared with miR‐NC group. (C) Relative expression of miR‐148a in HNT tumor and normal adjacent tissues. **P < 0.01 compared with adjacent group. (D) The linear analysis revealed a negative correlation between miR‐148a and HOTAIRM1 expression in HNT tissues (n = 109). (E) qRT‐PCR analysis of miR‐148a expression in Fadu cells transfected with p‐NC, p‐HOTAIRM1, HOTAIRM1 siRNA1, and HOTAIRM1 siRNA2. (F) qRT‐PCR analysis of miR‐148a expression in isolated tumor tissues from mice models. **P < 0.01 compared with miR‐NC group.