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. 2018 Jul 3;2018:7463832. doi: 10.1155/2018/7463832

Table 1.

Primer sequences, annealing temperatures, and genetic variations.

Polymorphisms Primer sequences Annealing temperatures Location Fragment (bp)
rs121434592
E17K
FW 5'- GGC CAA GGG GAT ACT TAC GC -3' 60 Exon 4 198
RV 5'- AGG GTC TGA CGG GTA GAG TG -3'

rs11555432
L357P
FW 5'- CCT TCT TGA GCA GCC CTG AA -3' 56.5 Exon 12 142
RV 5'- TAC GAG ATG ATG TGC GGT CG -3'

rs12881616
E319G
FW 5'- CAG AGA GGA CAC AGC ATT GCG -3' 60.5 Exon 10 186
RV 5'- ACA AGG ACG GGC ACA TTA AGA -3'

rs11555431
P388T
FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' 61 Exon 11 171
RV 5'- AAG TCC TTG CTT TCA GGG CT -3'

rs3803304 FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' 61 Intron 13
171
RV 5'- AAG TCC TTG CTT TCA GGG CT -3'

rs2494732 FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' 61 Intron 13 171
RV 5'- AAG TCC TTG CTT TCA GGG CT -3'

bp, base pairs.