Table 1.
Polymorphisms | Primer sequences | Annealing temperatures | Location | Fragment (bp) |
---|---|---|---|---|
rs121434592 E17K |
FW 5'- GGC CAA GGG GAT ACT TAC GC -3' | 60 | Exon 4 | 198 |
RV 5'- AGG GTC TGA CGG GTA GAG TG -3' | ||||
| ||||
rs11555432 L357P |
FW 5'- CCT TCT TGA GCA GCC CTG AA -3' | 56.5 | Exon 12 | 142 |
RV 5'- TAC GAG ATG ATG TGC GGT CG -3' | ||||
| ||||
rs12881616 E319G |
FW 5'- CAG AGA GGA CAC AGC ATT GCG -3' | 60.5 | Exon 10 | 186 |
RV 5'- ACA AGG ACG GGC ACA TTA AGA -3' | ||||
| ||||
rs11555431 P388T |
FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' | 61 | Exon 11 | 171 |
RV 5'- AAG TCC TTG CTT TCA GGG CT -3' | ||||
| ||||
rs3803304 | FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' | 61 | Intron 13 |
171 |
RV 5'- AAG TCC TTG CTT TCA GGG CT -3' | ||||
| ||||
rs2494732 | FW 5'- CAA GGA CAT CAA GCT TTG GCT -3' | 61 | Intron 13 | 171 |
RV 5'- AAG TCC TTG CTT TCA GGG CT -3' |
bp, base pairs.