Fig. 3.
The psm1K536A K1200A mutant is viable but sensitive to DNA-damaging agents. (A) Tetrads were dissected from the indicated diploid strains on YES plates and after four days at 32°C, spore viability was determined. Colonies were replica-plated onto selective YES plates containing hygromycin B (200 mg/l) or clonat (100 mg/l). (B) Cells were grown on YES medium for 1 day, diluted in 5-fold steps, spotted onto YES plates containing the indicated amounts of MMS or CPT and incubated for 3 days at 30°C.