Fig. 3.

MASK physically associates with Dome. (A) Schematic representation of Drosophila MASK protein and constructs used in this study. (B) Immunoprecipitation (IP) of the indicated HA–MASK constructs from Kc₁₆₇ cells also expressing Dome–FLAG (arrows). Dome–FLAG is co-immunoprecipitated with HA–MASK-A1 and HA–MASK-A2. Levels of Dome–FLAG present in the input lysate are shown in the lower panel. NS, non-specific band. (C) Co-precipitation of MASK-A1/A2-FLAG following immunoprecipitation of Dome–HA. Levels of MASK-A1/A2-FLAG, Dome-HA and α-Tubulin present in the total Kc₁₆₇ cell lysates are shown in the lower panels. (D) Steady-state levels of Dome–FLAG expressed in Drosophila Kc₁₆₇ cells are increased following the co-expression of HA–MASK-A2. Levels of α-Tubulin indicate loading parity. (E) Steady-state levels of MASK-A1/A2–FLAG expressed in Drosophila Kc₁₆₇ cells are increased following the co-expression of Dome–HA (arrowhead). Levels of α-Tubulin indicate loading parity.