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. 2018 Jul 6;131(13):jcs209551. doi: 10.1242/jcs.209551

Fig. 4.

Fig. 4.

MASK function is conserved in human cells. (A) Schematic representation of protein structure of Drosophila (Dm) MASK and human (Hs) ANKHD1 with percentage identity between sequences indicated for the highlighted regions. (B) SOCS3 mRNA expression in HeLa cells transfected with indicated siRNA and following OSM stimulation as indicated. ***P<0.001 (one-way ANOVA with Dunnett's posthoc test). Results are mean±s.d. (n=3). (C) HeLa cell extracts treated with siRNA targeting ANKHD1 have reduced levels of phospho-STAT3 upon OSM stimulation, whereas total STAT3 levels are unaffected. Blots confirm knockdown of ANKHD1 levels. (D) A representative blot of HeLa cells treated with control siRNA or siRNA targeting ANKHD1 in (n=3). Silencing of ANKHD1 leads to a loss of both ANKHD1 protein and endogenous GP130 protein. By contrast, levels of LIFR are not changed. β-actin levels are unaffected. (E,F) HeLa cell extracts treated with siRNA targeting ANKHD1 have reduced HA–EpoR (G) and TpoR–HA (H) compared to controls. Blots confirm knockdown of ANKHD1 levels.