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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Cell Rep. 2017 Oct 24;21(4):1089–1101. doi: 10.1016/j.celrep.2017.10.011

Figure 3. Effect of siRNA-Mediated Depletion of Rab43 on the Surface Expression and Signaling of α2B-AR.

Figure 3

(A) Western blot analysis of Rab43 expression in HEK293 cells.

(B) Effect of Rab43 siRNA on the surface expression of stably expressed HA-α2B-AR, and the rescue of their effect by Rab43.

(C) siRNA-mediated knockdown of Rab43 in MCF-7 cells.

(D) Effect of siRNA-mediated knockdown of Rab43 on the surface expression of endogenous α2-AR in MCF-7 cells.

(E) Colocalization of α2B-AR with calnexin in cells transfected with siRNA targeting Rab43.

(F) Effect of Rab43 siRNA on α2B-AR-mediated ERK1/2 activation.

Data represent mean ± SE (n = 3–4) (B, D, and F). *p < 0.05 versus NT. The images are representative of 3 experiments. Scale bar, 10 µm.