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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Cell Rep. 2017 Oct 24;21(4):1089–1101. doi: 10.1016/j.celrep.2017.10.011

Figure 4. Rab43 Modulates the ER-Surface Transport of Multiple GPCRs.

Figure 4

(A) Effect of Rab43N131I on the surface transport of GPCRs. HEK293 cells were transfected with individual GPCRs together with dsRed-C1 (Ctrl) or dsRed-Rab43N131I, and the cell-surface expression of each receptor was measured by radioligand binding or flow cytometry.

(B) Effect of siRNA-mediated depletion of Rab43 on the surface transport of GPCRs.

(C) Effect of Rab43 on the subcellular localization of different membrane proteins. Scale bar, 10 µm.

(D) Deglycosylation of α2A-AR (top) and AT1R (bottom) by Endo H treatment. $ indicates Endo-H-resistant bands and $$ Endo-H-sensitive bands.

(E) Quantitative data showing the effect of Rab43 on the formation of Endo-H-resistant (left) and Endo-H-sensitive glycosylation (right).

Data represent mean ± SE (n = 3–5) (A, B, and E). *p < 0.05 versus Ctrl. See also Figure S5.