Table 3. The minor DNA detection limit in the artificial DNA mixtures for each marker.
| SNP-STRs | Detection ratios (minor: major) in different SNP subtypes of mixtures | Overall detection ratios (minor: major) | |||
|---|---|---|---|---|---|
| A | G | C | T | ||
| rs11222421-D11S4463 | 1:50 * | 1:100 | 1:50 | ||
| rs12423685-D12ATA63 | 1:50* | 1:20 | 1:20 | ||
| rs2325399-D6S1043 | 1:20 | 1:100 | 1:20 | ||
| rs1276598-D6S474 | 1:100 * | 1:20 | 1:20 | ||
| rs16887642-D7S820 | 1:20 | 1:20 | 1:20 | ||
| rs9531308-D13S317 | 1:50 | 1:100 * | 1:50 | ||
| rs188010-D17S974 | 1:100 | 1:50 | 1:50 | ||
| rs258112-D5S2800 | 1:100 * | 1:100 * | 1:100 | ||
* The minor DNA could be distinguished in a mixture of 1:1000 for these SNP subtypes when they were genotyped with separate SNP allele-specific primers in two reactions and 35 PCR cycles.