Fig 6. Effects of COX inhibitors or siRNAs on feline calicivirus (FCV) replication.

(A) CRFK cells were pretreated or post-treated with noncytotoxic doses of SC-560, NS398, and indomethacin. At 8 h post-infection (hpi) with FCV (MOI, 1 FFU/ml), the levels of viral capsid protein were determined by Western blot analysis. GAPDH was used as a loading control. (B) CRFK cells transfected with COX-1, COX-2, or scrambled (Scram) siRNAs were incubated with FCV (MOI, 1 FFU/ml) for 8 h. Harvested samples were processed for Western blot analysis to detect FCV capsid protein. GAPDH was used as a loading control. (C) CRFK cells were treated with conditions described immediately above, and the effect of the COX-2 inhibitor NS398, or COX-1, COX-2, and scrambled (Scram) siRNAs on viral capsid protein production was determined by confocal microscopy. Bar = 20 μM.