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. 2018 Jul 18;13(7):e0200726. doi: 10.1371/journal.pone.0200726

Table 1. Oligonucleotides used in this study.

Target gene Sequence (5’-3’) Region (nt) Size (bp) Reference Accession no.
MNV-1 (ORF1)
(RT-PCR)
F: ATGAGGATGGCAACGCCATC
R: ATGATCTCTATCTTCGGGGA
6–25
996–1015
1015 This study DQ285629.1
FCV (RdRp)
(RT-PCR)
F: CCGCTGTCCAAAATCTCTCA
R: TCAGTAATCAATTCCCTTAA
3901–3920
4661–4680
779 This study M86379.1
MNV-1 (ORF1)
(qPCR)
F: GTGCGCAACACAGAGAAACG
R: GCAGGAAGCTCAGCCCG
39–58
160–177
139 Taube et al., 2009 DQ285629.1
FCV (RdRp)
(qPCR)
F: ACATTTCCTCGGAAACCTCT
R: GGAGAAGGTTAGTGAAGGGA
4041–4060
4261–4280
239 This study M86379.1
Murine COX-1 F: TTACCCTGGAGATGACGGGT
R: GGTTTTCGTGGCTTGGCATT
1895–1914
2011–2030
136 This study NM_008969
Murine COX-2 F: CTTCGGGAGCACAACAGAGT
R: CACCTGAGCGGTTACCACTT
1067–1086
1202–1221
155 This study NM_011198.4
Feline COX-1 F: GCAGTTGAGCGGTTACTTCC
R: CGGGAGTACAGCTACGAGC
1134–1153
1282–1300
167 This study XM_006939439
Feline COX-2 F: AAACACTCGGGAACTTCGCA
R: CTTGCTGTTCCAACCCATGC
32–51
188–207
176 This study EF036473
Murine beta-actin F: TATAAAACCCGGCGGCGCA
R: CTTTGCAGCTCCTTCGTTGC
1–19
64–83
83 This study NM_007393
Feline beta actin F: TCCTGGGTATGGAGTCCTGT
R: TCTACGCTAACACGGTGCTG
609–628
690–709
101 This study AB051104