Table 1. Oligonucleotides used in this study.
Target gene | Sequence (5’-3’) | Region (nt) | Size (bp) | Reference | Accession no. |
MNV-1 (ORF1) (RT-PCR) |
F: ATGAGGATGGCAACGCCATC R: ATGATCTCTATCTTCGGGGA |
6–25 996–1015 |
1015 | This study | DQ285629.1 |
FCV (RdRp) (RT-PCR) |
F: CCGCTGTCCAAAATCTCTCA R: TCAGTAATCAATTCCCTTAA |
3901–3920 4661–4680 |
779 | This study | M86379.1 |
MNV-1 (ORF1) (qPCR) |
F: GTGCGCAACACAGAGAAACG R: GCAGGAAGCTCAGCCCG |
39–58 160–177 |
139 | Taube et al., 2009 | DQ285629.1 |
FCV (RdRp) (qPCR) |
F: ACATTTCCTCGGAAACCTCT R: GGAGAAGGTTAGTGAAGGGA |
4041–4060 4261–4280 |
239 | This study | M86379.1 |
Murine COX-1 | F: TTACCCTGGAGATGACGGGT R: GGTTTTCGTGGCTTGGCATT |
1895–1914 2011–2030 |
136 | This study | NM_008969 |
Murine COX-2 | F: CTTCGGGAGCACAACAGAGT R: CACCTGAGCGGTTACCACTT |
1067–1086 1202–1221 |
155 | This study | NM_011198.4 |
Feline COX-1 | F: GCAGTTGAGCGGTTACTTCC R: CGGGAGTACAGCTACGAGC |
1134–1153 1282–1300 |
167 | This study | XM_006939439 |
Feline COX-2 | F: AAACACTCGGGAACTTCGCA R: CTTGCTGTTCCAACCCATGC |
32–51 188–207 |
176 | This study | EF036473 |
Murine beta-actin | F: TATAAAACCCGGCGGCGCA R: CTTTGCAGCTCCTTCGTTGC |
1–19 64–83 |
83 | This study | NM_007393 |
Feline beta actin | F: TCCTGGGTATGGAGTCCTGT R: TCTACGCTAACACGGTGCTG |
609–628 690–709 |
101 | This study | AB051104 |