Figure 3.

Baseline behavioral analysis of naïve Syn1creIKK2^fl/fl and IKK2^fl/fl mice. (A, B) Rotarod performance under naïve conditions. A learning component is observed in both genotypes (two-way RM ANOVA), but Syn1creIKK2^fl/fl mice spent significantly less time on the rod than littermates during each trial (T1-4) (two-way ANOVA, post hoc multiple t-test) (A) and in total (student’s t-test) (B) demonstrating impaired motor coordination (n=6 mice/group). (C) Hargreaves test showing comparable latency to remove paws between IKK2^fl/fl and Syn1creIKK2^fl/fl mice under naïve conditions (n=6 mice/group). (D) Grip strength (g, gram) analysis showed comparable neuromuscular function in naïve IKK2^fl/fl and Syn1creIKK2^fl/fl mice (n=16 mice/group). (E–G) Elevated plus maze test showed that IKK2^fl/fl and Syn1creIKK2^fl/fl mice spent comparable amounts of time in the open (E) and closed (F) arms and travelled a similar total distance during the trial (G) (n=12–13 mice/group). (I–K) Open field test showed that Syn1creIKK2^fl/fl mice travelled a significantly longer distance than IKK2^fl/fl mice in the open field (student’s t-test) (H), whereas number of zone changes (I), center/perimeter ratio (J)were comparable between genotypes,and number of wall rearing (K) was significantly decreased for Syn1creIKK2^fl/fl mice compared to IKK2^fl/fl mice, n=6 mice/group. All data are presented as mean±SEM. *p<0.05, **p<0.01, ^###p<0.001 ****p<0.0001.