Fig. 1. Microfluidics-based in vitro cell-rolling assay for characterizing HSPC rolling.

(A) Experimental configuration of the microfluidics SR microscopy platform. (B) Transmitted light microscopy images of KG1a cells rolling on E-selectin. The surface density of the E-selectin was 3.6 molecules μm⁻². (C) Wall shear stress–dependent rolling velocity of KG1a cells (blue) and the number of bound cells to the E-selectin surface (red). Error bars indicate SDs of six different fields of view. (D) E-selectin density–dependent rolling velocity of KG1a cells (blue) and the number of bound cells to the E-selectin surface (red). Error bars indicate SDs of six different fields of view. CCD, charge-coupled device; EMCCD, electron multiplying CCD.