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. Author manuscript; available in PMC: 2019 May 16.
Published in final edited form as: Bioconjug Chem. 2018 Apr 5;29(5):1646–1658. doi: 10.1021/acs.bioconjchem.8b00144

Figure 3. Enrichment of stable, EGFR-binding charge mutants.

Figure 3

(A) Yeast libraries displaying charge-modulated Gp2 ligands were sorted against biotinylated EGFR ectodomain (2 – 20 nM) after pre-heating the yeast for 30 minutes (60–80 °C) followed by screening against streptavidin- and mIgG-coated beads to deplete non-specific binders, and finally sorted against lysate-extracted EGFR at 2 and 0.2 nM target. EGFR-labeled and target-negative yeast are shown in red and black with collected populations in triangular gates.