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. Author manuscript; available in PMC: 2019 Jul 9.
Published in final edited form as: ACS Comb Sci. 2018 Jun 5;20(7):423–435. doi: 10.1021/acscombsci.8b00010

Figure 5. Allowance of C, G, R, and W aids Gp2 library efficacy in loop 1 and hinders efficacy in loop 2.

Figure 5

Libraries either had high diversity at each paratope site based on the antibody CDR (base) or had multiple sites constrained to lower diversity (constrained). The evaluation of a cysteine-free library (and limitations of degenerate nucleotides) resulted in libraries that contained (+) or lacked (−) the amino acids C, G, R, and W at certain sites. Change in sequence frequencies from before (white) and after (grey) evolution were compared between libraries.