Skip to main content
. Author manuscript; available in PMC: 2019 Jul 9.
Published in final edited form as: ACS Comb Sci. 2018 Jun 5;20(7):423–435. doi: 10.1021/acscombsci.8b00010

Figure 6. A particular sitewise amino acid constraint hinders Gp2 binder discovery.

Figure 6

Enrichment in sequence frequency from the combined naïve library to evolved binders is shown for libraries where nine paratope sites were constrained (red) based on first generation binder and natural homolog sequences, computational stability, and solvent accessibility, or where these sites were left as full diversity CDR (white with black lines). The CDR diversity was either cysteine-free (CDR−) or contained all amino acids (CDR+).