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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Methods. 2018 Feb 16;143:102–109. doi: 10.1016/j.ymeth.2018.02.014

Figure 7.

Figure 7

Effect of SAM on biosensor and Spinach2 fluorescence. (a) In vitro fluorescence activation of (a) biosensor Pi-4–5 and (b) Spinach2 at 30 °C, 10 mM MgCl2 and 37 °C, 3 mM MgCl2 in the presence of 0, 50, 500, and 1000 µM SAM. Data shown are mean values ± standard deviation taken from two independent replicates.