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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Methods. 2018 May 23;144:94–103. doi: 10.1016/j.ymeth.2018.05.016

Figure 4.

Figure 4

Comparison of oxidation extent on calmodulin without any treatment (pink), with H2O2 and laser off (blue), with H2O2 and laser on (green). Charge state +14 is shown. FPOP experiment parameters: calmodulin 2 μM, histidine 2 μM, H2O2 20 mM, laser energy 24 mJ/pulse, spot width 2.2 mm, shot rate 7.2 Hz, capillary I.D. 150 μm, flow rate calculated and set as 22.4 μL/min, E.V. 25%.