Figure 3.
DKK1 methylation patterns in cancer cells. A 233 bp CpG island encompasses the transcriptional start site and a portion of the first exon of DKK1. Eighteen potential methylation sites were identified within this CpG island. Methylation-specific sequencing was performed to identify the methyl-deoxycytidines within this CpG island. The COLO205 colon cancer cell line served as a control for methylation as this region of the DKK1 gene was reported methylated [19].