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Demethylation restored DKK1 expression in C4-2B prostate cancer cells. Cancer cell lines were treated with and without 10 μM of 5-aza-2′-deoxycytidine (5-dAza-C) for 4 days. Messenger RNA was isolated from the cells and subjected to DKK1 real-time RT PCR. (NS = not significant; * = P < .05; *** = P < .001).
