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Expression of DKK1 binding partners and Wnt signaling inhibitors. Real-time RT PCR was performed in seven cancer cell lines that produce bone lesions in animal models of bone metastasis. Messenger RNA was analyzed for the genes that encode for LDL-related receptor proteins 5 and 6 (LRP5, LRP6), Sclerostin (SOST), mesoderm development candidate 2 protein (MESD), Kremen1 (Krm1) and Kremen2 (Krm2).
