Fig. 5.

Inhibition of EZH2 T367 phosphorylation reduces breast cancer metastasis. a Representative bioluminescence images of primary tumors at one and nine weeks post tumor implantation. b Primary tumor growth curves of NOD/SCID mice orthotopically-implanted with MDA-MB-231 EZH2 knockdown rescue cells (n = 10 per condition) expressing GFP-Luciferase. Primary tumor growth as determined by caliper measurements, shown as mean ± SD. c Representative bioluminescence images of metastases (primary tumor shielded), imaged at four weeks post tumor implantation (left) with representative H&E staining of lung tissue from each of the four groups at nine weeks post implantation (right, ×600 magnification). d Metastatic lung burden assessed by measuring photon flux measured four weeks post tumor implantation using Live Image Pro after shielding primary tumors. Data are presented as means ± SEM. e Kaplan–Meier metastasis-free survival curve of mice as determined by presence of lung metastases with bioluminescence imaging showing difference between WT-EZH2 (red) and T367A-EZH2 (blue) knockdown-rescue groups. *p ≤ 0.05; **p ≤ 0.01