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. 2018 Jul 18;8:10879. doi: 10.1038/s41598-018-29001-9

Figure 3.

Figure 3

Drp1 VD directs helical self-assembly. (A) SEC elution profiles of purified Drp1 WT and Drp1ΔVD each loaded at 6 μM onto a Superose 6 10/300 GL column. (B) Representative EM images of Drp1ΔVD polymers upon spin-sedimentation at 20,800×g (left) and in suspension (right). Scale bar, 200 nm. Inset, a magnified image of non-helical Drp1ΔVD polymers. Scale bar, 50 nm. (C) SEC-MALS profiles of the included fraction of Drp1ΔVD (~70 kDa monomer) loaded at 6 μM onto a Superose 6 10/300 GL column. SEC-MALS revealed that Drp1ΔVD formed tetramers (~70 × 4 = ~280 kDa) in solution.