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. 2018 Jul 18;8:10879. doi: 10.1038/s41598-018-29001-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Drp1 VD is monomeric and does not directly participate in self-assembly. (A) Secondary structure prediction of the Drp1 VD sequence determined by PSIPRED⁸⁴. (B) SEC elution profiles of GST-excised, tag-free Drp1 VD loaded at 75 and 200 μM onto a Superdex 75 10/300 GL column. (C) CD spectrum of 200 μM 6X His-tagged Drp1 VD. Inset, CD spectrum of 17.2 μM MffΔTM is shown for comparison. (D) SEC elution profiles of tag-free Drp1 VD (200 μM) and Dyn1 PH domain (40 μM; ~14 kDa monomer) loaded onto a Superdex 75 10/300 GL column. (E) SEC-MALS profiles of dimeric MffΔTM (~27 kDa monomer × 2) and dimeric GST (~25 kDa monomer × 2) loaded onto a Superose 6 10/300 GL column. (F) SEC elution profile of monomeric MffΔTMΔCC (~22 kDa)³⁶ relative to Drp1 VD, loaded at 75 and 45 μM, respectively, onto a Superose 6 10/300 GL column.