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. 2018 Jul 18;8:10849. doi: 10.1038/s41598-018-29123-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The enrichment of persisters with both S. aureus Newman wild type and transposon library. 24-hour culture of either wild type (WT 1–3) or Tnp library (Lib 1–3) was exposed to 100 × MIC of ciprofloxacin (MIC = 0.5 µg/ml) for another 24 hours before plating on TSA plates for CFU calculation. The same procedure was repeated consecutively for 4 times (Round 1–4) and the persister frequencies were recorded accordingly.