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. 2018 Jul 18;8:10849. doi: 10.1038/s41598-018-29123-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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pH does not affect persister formation. (a) pH of cell-free supernatant. Wild type (WT) and ΔsucA were cultivated in TSB medium with or without glucose (TSB-glucose) for 24 hours and the pH of the supernatant was measured and compared; (b) the persister frequency of wild type (WT) and ΔsucA was calculated when cultivated with TSB medium or TSB medium without glucose (TSB-glucose). There were 3 biological replicates for each sample and error bars stand for standard deviation. Starting CFU/ml are indicated in Supplementary Table S2.