Figure 5.
ATP is not the sole determinant of cellular persistence. (a) The ATP content of TCA cycle mutants (acnA, citC, sucA, sucB, sucD, sdhA, sdhB and ΔsucA) was measured after 24 hours of cultivation; (b) the ATP levels of wild type (WT) and transductants of Tnp library isolates (L11-L61) were measured after 24-hour cultivation. There were 3 biological replicates for each sample and 3 different reads of luminescence signal for each biological replicate. The statistical comparison between wild type and TCA cycle mutants (a) or Tnp isolate transductants (b) was determined with one way ANOVA followed by Dunnett’s test. The error bars stand for standard deviation and the asterisks indicate significant difference at P < 0.05.