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. 2018 Jul 18;8:10862. doi: 10.1038/s41598-018-29190-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Annotation of Border Collie de novo contigs. (a) Flow chart of contig annotation. Number of contigs is indicated in red, contigs preferentially aligning to dog, human, or mouse protein Ensembl databases are indicated by colored boxes. Note: Some contigs aligned with equal weight to two databases. (b) Venn diagram of the number of protein coding genes to which novel contigs aligned. Numbers in the overlapping regions indicate the number of BLASTx hits aligning to dog (red), human (yellow), and mouse (blue).