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. 2018 Jul 18;8:10847. doi: 10.1038/s41598-018-29165-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exclusion chromatography of tomato root exudate (TRE) and Medicago root exudate (MRE). The four chromatograms are from separate runs on the same Bio-Gel P2 column. Chromatography was of (a) vitamin B12 and TRE, (b), dextran 40, vitamin B12, raffinose and ribose, (c) vitamin B12 and MRE, and (d) dextran 40, vitamin B12 and maltose. Attraction assay results are presented as a chemotaxis index, CI. Results from standards are concentrations expressed as a fraction of the applied concentration. For attraction assays, each bar represents the average of 3 replicate assays with SD shown.