Table 1.
The gene expressing cassettes used in this study.
| Gene | Species | Function | Promoter | terminator | Cassette size (bp) |
|---|---|---|---|---|---|
| crtE | Xanthophyllomyces dendrorhous | Geranylgeranyl pyrophosphate synthase | ScGapDH | ScGapDH | 2,083 |
| crtI | Xanthophyllomyces dendrorhous | Phytoene desaturase | ICL | 35 S | 3,935 |
| crtYB | Xanthophyllomyces dendrorhous | Phytoene synthase/lycopene cyclase | ScPGK | ScPGK | 3,186 |
| tHMG1 | Kluyveromyces marxianus | Truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase | ScADHI | ScADHI | 2,911 |
| CBHI | Trichoderma reesei | Cellobiohydrolases | KlADHI | ScGapDH | 2,680 |
| CBHII | Trichoderma reesei | Cellobiohydrolases | KlPGK | ScPGK | 2,502 |
| EgIII | Trichoderma reesei | Endo-β-1,4-glucanases | ScGapDH | ScGapDH | 2,161 |
| EgI | Aspergillus niger | Endo-β-1,4-glucanases | ScPGK | ScPGK | 2,731 |
| EglA | Aspergillus niger | Endo-β-1,4-glucanases | Lac4 | Lac4 | 2,925 |
| BGS | Neocallimastix patriciarum | β-glucosidases | ScADHI | ScADHI | 3,691 |
| KanMx | — | Geneticin (G418) resistance | KlGapDH | ScGapDH | 1,855 |
| Sh ble | — | Zeocin resistance | ScADHI | ScADHI | 1,786 |
| hph | — | Hygromycin B resistance | ScADHI | ScADHI | 2,437 |
| Total size: | 34,890 |