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. 2018 Jul 18;9(8):791. doi: 10.1038/s41419-018-0824-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The proliferation of HeLa and SiHa cell lines was analyzed using a colony-formation assay after transfection with BAP31 or control siRNA. The results represented the means ± SD of triplicate independent experiments. The scale bars represent 100 μm. b Flow cytometric analysis of the HeLa and SiHa cell cycle distributions with PI at 48 h post transfection with BAP31 or control siRNA. The results are shown as the percentages of cells in G1, S, and G2 phases. *p < 0.05 was considered to be significantly different for the siBAP31 vs siCon group. c Western blotting results for the expression levels of BAP31, Cyclin D1, Cyclin E1, and Cyclin E2 in the control and the BAP31-depleted group of HeLa and SiHa cell lines. d Wound-healing assays were performed to evaluate the migration of HeLa and SiHa cells transfected with BAP31 or control siRNA. The scale bars represent 100 μm. e Transwell invasion assay of HeLa and SiHa cells treated as in (a). The results represent the means ± SD from five microscopic fields. The scale bars represent 100 μm. *p < 0.05 and **p < 0.01 were considered significantly different for the siBAP31 vs siCon groups