Fig. 5. Depletion of BAP31 suppresses cervical cancer progression and metastasis in vivo.

a Image of tumors and tumor volumes measured after 30 days. HeLa cells were stably transfected with BAP31 shRNA and injected subcutaneously into the left and right flanks of nude mice. HeLa cells transfected with control shRNA were used as controls and five mice were included in each group. The results represent the mean ± SD and **P < 0.05 was considered to be significantly different using Student’s t test. b Growth curve of xenografts of Hela cells transfected with BAP31 shRNA or Scramble shRNA. (n = 5, Mean ± SD) c Kaplan–Meier means curve representing the overall survival of the injected mice with BAP31-depleted or control HeLa cells. (p < 0.01 by log-rank test). d Representative pictures and statistics analysis of the tumor metastasis in livers. Arrows indicate tumor metastasis site. The results represent the mean ± SD and *P < 0.05 was considered to be significantly different using Student’s t test. e Immunohistochemical staining of BAP31 in tumor tissue from xenograft mouse model and detection of liver metastasis by HE staining. The scale bars represent 1 mm and 300 μm