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. 2018 Jul 18;8:10843. doi: 10.1038/s41598-018-29203-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of triptolide on podocin expression and distribution in MTZ-treated Tg(pod:gal4;UAS:NTR-mCherry) zebrafish. (A) The expression and distribution of podocin in different groups was measured and examined at 24 h, 30 h and 36 h after MTZ treatment in 84 hpf larvae; (a1–a3) normal control (CTL); (b1–b3) MTZ model group; (c1–c3) MTZ + triptolide group. (B) No obvious mean immunofluorescence intensity (GL) differences were observed between the three groups at 30 h and 36 h. (C) Positively stained area to the glomerular area (AR) revealed a remarkably reduction at 36 h in the MTZ model group, but remained strong in the triptolide treatment group. (n = 3), **P < 0.01, (original magnification, x1000).