Figure 1.
Raw thermograms and calculated thermodynamic characteristics of ITC experiments conducted with the holothuroid saponin Frondoside A and various liposome compositions. (A) Holothuroid-like liposomes composed of DMPC:D(C16:1)PC: Δ7 sterol (5:2:3) (B) Sterol free liposomes composed of DMPC:D(C16:1)PC (3:1) (C) and fish-like liposomes D(C16:1)PC:DOPC:Chol (5:2:3). Graphic representation of the thermodynamic values (D) Enthalpy (E) Entropy (F) Free Gibbs Energy, associated with the different ITC experiments. Error bars are standard deviation of replicates (n = 3). Lowercase letters depict significant differences calculated through a pairwise comparison using t-test.