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. 2018 Jul 12;6:95. doi: 10.3389/fbioe.2018.00095

Figure 3.

Figure 3

Expression analysis of the PCC7942-NSII-CotA and wild type PCC7942-WT: (A) RT-PCR products confirming expression of the laccase in strain PCC7942-NSII-CotA. Strains and controls labeled in convention Strain ID: Forward primer ID_Reverse primer ID. Strain IDs and primer IDs can be found in Table 1. Size of the CotA laccase gene is 1539 bp, and C-phycocyanin beta subunit gene, that was used as positive control, 519 bp. M, molecular weight marker. (B) SDS-PAGE of the CotA expressing S. elongatus PCC7942-NSII-CotA. Total cell extract of PCC7942-NSII-CotA and PCC7942-WT were fractionated by SDS/PAGE and stained with EZBlue™ Gel Staining Reagent. Lanes: PCC7942-WT: wild-type S. elongatus PCC7942; PCC7942-NSII-CotA: NSII-targeted, CotA-expressing transformant of S. elongatus PCC7942, M, molecular weight marker, Arrow indicates CotA band confirmed by peptide sequencing.