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. 2018 Jul 12;6:95. doi: 10.3389/fbioe.2018.00095

Figure 4.

Figure 4

Decolorization of tested dyes (0.1 g/l) by cell-free culture medium containing CotA laccase produced by the recombinant cyanobacterium PCC7942-NSII-CotA (light gray bars) and by the cell-free culture medium of the control strain PCC7942-WT (dark gray bars) during 7 days of incubation. Following decolorization conditions were used: (A) No ABTS mediator in 0.05M phosphate-citric acid buffer (pH 5.5), (B) 10 mM (final) ABTS mediator in 0.05M phosphate-citric acid buffer (pH 5.5); (C) No ABTS mediator in 0.05M phosphate-citric acid buffer (pH 7.5); (D) 10 mM ABTS mediator in in 0.05M phosphate-citric acid buffer (pH 7.5). Abbreviations: Reactive Blue 19 (RB19); Reactive Blue 4 (RB4); Reactive Black 5 (RB5), Acid Red 18 (AR18), Acid Red 27 (AR27), Acid Yellow 18 (AY18), Reactive Red 11 (RR11) Reactive Orange 5 (RO5), and Indigo carmine (IC).