Table 1.

PCR primers used in this study.

Primer name	Sequence 5′ → 3′	Annealing temperature	Amplicon length	Reference
CotAF	ATGCCTGCAGGTCGACGATA	52	1539 bp	This study
CotAR	TTATTTATGAGGATCAGTAATATCCATAGGT
CotA-veF	ATAACCCAGGGATTTATGCCTGCAGGTCGACGAT	58	1569 bp	This study
CotA-veR	GGAGCTCCTTCATTTTTATTTATGAGGATCAGTAATATCCA
NSIILF	GCCACGATTTGAGGGACGAATC	55	WT: 1476 bp	This study
NSIIRR	ATCAGGATTAATGAAACGGACGC		NSII-CotA: 4400 bp
NSILF	CGGAGCGCTGCTTTCTTGG	55	WT: 1665 bp	This study
NSIRR	CGGAGCGCTGCTTTCTTGG		NSI-CotA: 4679 bp
PhycoBF	ATGCTAGATGCATTYRCCAARGTT	55	594 bp	This study
PhycoBR	TTAGGCWACNGCRGCAGCGGC	55	594 bp	This study