Table 2.

Plasmids and strains used in this study.

Plasmid ID	Relevant characteristics	Reference
pCV0062	Plasmid contains comprises: the chromosome integration site NSII for Synechococcus elongatus PCC7942; origin of replication for E. coli derived from pBR322 and an oriT site for conjugal transfer; aadA promoter and gene that confers spectinomycin and streptomycin resistance; the ccdB gene (toxic for commonly used E. coli cell lines), which can be taken out with the SwaI nuclease.	Taton et al., 2014
pCV0063	Plasmid contains comprises: the chromosome integration site NSI for Synechococcus elongatus PCC7942; origin of replication for E. coli derived from pBR322 and an oriT site for conjugal transfer; aadA promoter and gene that confers spectinomycin and streptomycin resistance; the ccdB gene (toxic for commonly used E. coli cell lines), which can be taken out with the SwaI nuclease.	Taton et al., 2014
pCV0062-CotA	pCV0062 plasmid digested with SwaI nuclease, with codon-optimized CotA laccase inserted in SwaI digested site	This study
pCV0063-CotA	pCV0062 plasmid digested with SwaI nuclease, with codon-optimized CotA laccase inserted in SwaI digested site
Strain ID
PCC7942-WT	S. elongatus PCC 7942 wild type	Collection Pasteur
PCC7942-NSI-CotA	S. elongatus PCC 7942 NSI: PaadA-aadA-PconII-CotA	This study
PCC7942-NSII-CotA	S. elongatus PCC 7942 NSII: PaadA-aadA-PconII-CotA	This study