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. 2018 Jul 18;8:10888. doi: 10.1038/s41598-018-29239-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EHMT1 and EHMT2 are required for accumulation of ubiquitin conjugates and repair factors at DNA damage sites. (a,b) Immunofluorescence analysis of U2OS cells (a) and MCF7 (b) cells transfected with indicated siRNA, and co-immunostained with indicated antibodies at 2 h after exposure to neocarzinostatin (NCS, 50 ng/ml for 15 min). A representative image of each treated or control cells is shown, as indicated. DNA damage induced foci are quantified as the percentage of cells with more than 5 large foci in nuclei after background subtraction, each based on at least 150 cells from three independent experiments (right). Error bars represent standard deviation (SD). Statistical significance was calculated using two-tailed, unpaired t-test compared with control cells; *P < 0.0001. The knockdown efficiencies with individual siRNAs against EHMT1 and EHMT2 are shown in Fig. S3a,b. Scale bar, 10 μm.