Fig. 1. Wnt/β-catenin signaling is expressed in M2 macrophages.

a, b BMDMs (M0) were stimulated with LPS + IFN-γ (M1) or IL-4 (M2). The expression of receptors (a) and the downstream genes of Wnt signaling (b) were determined by qRT-PCR, using β-actin as an internal control. A quantitative comparison among M0, M1, and M2 macrophages was presented (n = 5). c The protein level of downstream genes of Wnt signaling including β-catenin and c-Myc was detected by western blotting, and then quantified among M0, M1, and M2 macrophages (n = 3). d The expression of β-catenin in different polarized BMDMs was detected by immunofluorenscence (IF) staining. Cell nuclei were counterstained with Hoechst (n = 3). Bars, mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001