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. 2018 Jul 18;9:2812. doi: 10.1038/s41467-018-05097-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Tbkbp1 mediates IL-15-stimulated NKT cell survival. a, b Flow cytometric analysis of NKT cell frequency in NKT-enriched WT and Tbkbp1-KO thymocytes (CD8⁺ cells depleted by microbeads) after in vitro incubation with IL-15 for the indicated time periods. Data are presented as a representative plot (a) and summary graph (b). c, d Flow cytometry analysis of apoptotic cells (based on AnnexinV and PI staining) in enriched thymic NKT cells cultured for indicated time periods with IL-15 or medium control. Data are presented as a representative plot (c) and summary graph (d). e Proliferation assays (based on CFSE dilution) of WT or Tbkbp1-KO thymic NKT cells labeled with CFSE and cultured for the indicated time with IL-15. f, g Flow cytometric analysis of intracellular Bcl-2 level (f) and qRT-PCR analysis of Bcl2 mRNA (g) in WT or Tbkbp1-KO thymic NKT cells, either freshly isolated or cultured with IL-15 for 48 h, presented as a representative plot (left) and summary graph (right). All summary graphs are presented as mean ± s.d. values based on 5 WT and 5 Tbkbp1-KO mice. The similar data were obtained from at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. Mann–Whitney test (b), Student’s t-test (d, g)